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Phospho-CARM1 (Ser228) Antibody

PACO24363
Assay Genie
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCARM1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Phospho-CARM1 (Ser228) Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA, WB, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID10498
  • Target name
    CARM1
  • Target description
    coactivator associated arginine methyltransferase 1
  • Target synonyms
    PRMT4, histone-arginine methyltransferase CARM1, protein arginine N-methyltransferase 4
  • Host
    Rabbit
  • Protein IDA6NN38
  • Protein Name
    Histone-arginine methyltransferase CARM1
  • Scientific Description
    Phospho-CARM1 (Ser228) Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IF, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human, Mouse, Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100ul format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of CARM1 using anti-CARM1 antibody (A01486-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARM1 antigen affinity purified polyclonal antibody (Catalog # A01486-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARM1 at approximately 66 kDa. The expected band size for CARM1 is at 66 kDa.
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