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Phospho-CDC37-S13 Rabbit mAb

AP1141
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDC37
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Overview

  • Supplier
    ABclonal
  • Product Name
    Phospho-CDC37-S13 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID11140
  • Target name
    CDC37
  • Target description
    cell division cycle 37, HSP90 cochaperone
  • Target synonyms
    P50CDC37, hsp90 co-chaperone Cdc37, CDC37 (cell division cycle 37, S. cerevisiae, homolog), CDC37 cell division cycle 37 homolog, cell division cycle 37 homolog, hsp90 chaperone protein kinase-targeting subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16543
  • Protein Name
    Hsp90 co-chaperone Cdc37
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cdc37 using anti-Cdc37 antibody (PB9574). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: 293T Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc37 antigen affinity purified polyclonal antibody (Catalog # PB9574) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc37 at approximately 44 kDa. The expected band size for Cdc37 is at 44 kDa.
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