A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-EGFR(Y1068)) Monoclonal Antibody, Unconjugated (bsm-54587R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-EGFR(Y1068)) Monoclonal Antibody, Unconjugated (bsm-54587R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Phospho-EGFR(Y1068) Monoclonal Antibody
BSM-54587R
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetEGFR
Overview
- SupplierBioss Antibodies
- Product NamePhospho-EGFR(Y1068) Monoclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- Applications SupplierWB(1:300-5000), FCM(1:20-100), IF(ICC)(1:50-200), IP(1-2ug)
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1H2
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID1956
- Target nameEGFR
- Target descriptionepidermal growth factor receptor
- Target synonymsavian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; ERRP; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
- HostRabbit
- IsotypeIgG
- Protein IDP00533
- Protein NameEpidermal growth factor receptor
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203