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Western Blot analysis of JK cells using Phospho-FGFR-4 (Y642) Polyclonal Antibody
Western Blot analysis of JK cells using Phospho-FGFR-4 (Y642) Polyclonal Antibody
Western Blot analysis of JK cells using Phospho-FGFR-4 (Y642) Polyclonal Antibody

Phospho-FGFR4 (Y642) Antibody

CSB-PA008250
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFGFR4
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-FGFR4 (Y642) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2264
  • Target name
    FGFR4
  • Target description
    fibroblast growth factor receptor 4
  • Target synonyms
    CD334, JTK2, TKF, fibroblast growth factor receptor 4, hydroxyaryl-protein kinase, protein-tyrosine kinase, tyrosine kinase related to fibroblast growth factor receptor, tyrosylprotein kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22455
  • Protein Name
    Fibroblast growth factor receptor 4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FGFR4 using anti-FGFR4 antibody (PB9193). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human FGFR4 protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR4 antigen affinity purified polyclonal antibody (Catalog # PB9193) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR4 at approximately 39 kDa. The expected band size for FGFR4 is at 39 kDa.
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Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
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