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Phospho-HMGN2 (S29) Antibody

CSB-PA009217
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetHMGN2
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-HMGN2 (S29) Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3151
  • Target name
    HMGN2
  • Target description
    high mobility group nucleosomal binding domain 2
  • Target synonyms
    high mobility group nucleosome-binding domain-containing protein 2; high mobility group protein N2; high-mobility group (nonhistone chromosomal) protein 17; HMG17; non-histone chromosomal protein HMG-17; nonhistone chromosomal protein HMG-17
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05204
  • Protein Name
    Non-histone chromosomal protein HMG-17
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HMGN2 using anti-HMGN2 antibody (A04839-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGN2 antigen affinity purified polyclonal antibody (Catalog # A04839-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMGN2 at approximately 18 kDa. The expected band size for HMGN2 is at 18 kDa.
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