Western Blot Positive WB detected in HepG2 whole cell lysate(treated with Calyculin A or not) All lanes Phospho-STAT1 antibody at 1.065microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 87 KDa Observed band size: 87 KDa
Western Blot Positive WB detected in HepG2 whole cell lysate(treated with Calyculin A or not) All lanes Phospho-STAT1 antibody at 1.065microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 87 KDa Observed band size: 87 KDa
Phospho-STAT1 (S727) Recombinant Monoclonal Antibody
CSB-RA022810A727PHHU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSTAT1
Overview
- SupplierCusabio
- Product NamePhospho-STAT1 (S727) Recombinant Monoclonal Antibody
- Delivery Days Customer20
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2H10
- ConjugateUnconjugated
- Gene ID6772
- Target nameSTAT1
- Target descriptionsignal transducer and activator of transcription 1
- Target synonymsCANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription 1-alpha/beta; STAT91; transcription factor ISGF-3 components p91/p84
- IsotypeIgG
- Protein IDP42224
- Protein NameSignal transducer and activator of transcription 1-alpha/beta
- Scientific DescriptionSignal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC12352203