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Western Blot analysis of JK cells using Phospho-Vav3 (Y173) Polyclonal Antibody
Western Blot analysis of JK cells using Phospho-Vav3 (Y173) Polyclonal Antibody
Western Blot analysis of JK cells using Phospho-Vav3 (Y173) Polyclonal Antibody

Phospho-VAV3 (Y173) Antibody

CSB-PA006523
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetVAV3
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-VAV3 (Y173) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10451
  • Target name
    VAV3
  • Target description
    vav guanine nucleotide exchange factor 3
  • Target synonyms
    guanine nucleotide exchange factor VAV3; vav 3 guanine nucleotide exchange factor; vav 3 oncogene; VAV-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKW4
  • Protein Name
    Guanine nucleotide exchange factor VAV3
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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WB (peptide competition) analysis of Cos7 cells over-expressing murine Vav3 were serum starved and left treated (Lane 1) or treated (Lane 2-5) with EGF using GTX24764 VAV3 (phospho Tyr173) antibody prior incubated with the non-phospho immunogen (Lane 3), a generic (pY) peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
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Figure 1. Western blot analysis of VAV3 using anti-VAV3 antibody (A03204-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAV3 antigen affinity purified polyclonal antibody (Catalog # A03204-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VAV3 at approximately 98 kDa. The expected band size for VAV3 is at 98 kDa.
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