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ProductsBack/Phospho-WRN (S1141) Antibody

Phospho-WRN (S1141) Antibody

CSB-PA060086
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetWRN
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-WRN (S1141) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7486
  • Target name
    WRN
  • Target description
    WRN RecQ like helicase
  • Target synonyms
    RECQ3, RECQL2, RECQL3, bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN, DNA helicase, RecQ-like type 3, Werner syndrome ATP-dependent helicase, Werner syndrome RecQ like helicase, Werner syndrome protein, Werner syndrome, RecQ helicase-like, exonuclease WRN, recQ protein-like 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14191
  • Protein Name
    Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WRN using anti-WRN antibody (PB10107). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WRN antigen affinity purified polyclonal antibody (Catalog # PB10107) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WRN at approximately 162kDa, 200 kDa. The expected band size for WRN is at 162 kDa.
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Immunofluorescent staining of human cell line U2OS shows localization to nuclear speckles.
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