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ProductsBack/Phospho-ZYX (S142) Antibody

Phospho-ZYX (S142) Antibody

CSB-PA060122
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetZYX
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-ZYX (S142) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7791
  • Target name
    ZYX
  • Target description
    zyxin
  • Target synonyms
    ESP-2, HED-2, zyxin, zyxin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15942
  • Protein Name
    Zyxin
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Zyxin using anti-Zyxin antibody (M02365). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Zyxin antigen affinity purified monoclonal antibody (Catalog # M02365) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Zyxin at approximately 78 kDa. The expected band size for Zyxin is at 61 kDa.
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