Phospholipase D (PLD) Assay Kit (Colorimetric/Fluorometric)

LifeSpan BioSciences

Phospholipase D (PLD) Assay Kit (Colorimetric/Fluorometric)

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For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.

Spectrophotometry (fluorometric), Spectrophotometry (visible) For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.

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Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers. This Method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.

-20°C

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