Phospholipid Assay Kit (Colorimetric/Fluorometric)

LifeSpan BioSciences

Phospholipid Assay Kit (Colorimetric/Fluorometric)

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For quantitative determination of phospholipid and evaluation of drug effects on its metabolism.

Spectrophotometry (fluorometric), Spectrophotometry (visible) For quantitative determination of phospholipid and evaluation of drug effects on its metabolism.

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Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. This Method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.

-20°C

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