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Western blot All lanes: PHPT1 antibody at 2microg/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa
Western blot All lanes: PHPT1 antibody at 2microg/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa
Western blot All lanes: PHPT1 antibody at 2microg/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa

PHPT1 Antibody

CSB-PA017942DA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPHPT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PHPT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29085
  • Target name
    PHPT1
  • Target description
    phosphohistidine phosphatase 1
  • Target synonyms
    CGI-202, HEL-S-132P, HSPC141, PHP, PHP14, 14 kDa phosphohistidine phosphatase, epididymis secretory sperm binding protein Li 132P, protein histidine phosphatase, protein janus-A homolog, sex-regulated protein janus-a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NRX4
  • Protein Name
    14 kDa phosphohistidine phosphatase
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PHPT1 using anti-PHPT1 antibody (A06841). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHPT1 antigen affinity purified polyclonal antibody (Catalog # A06841) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHPT1 at approximately 14 kDa. The expected band size for PHPT1 is at 14 kDa.
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