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Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-PI3KCA Polyclonal Antibody, Unconjugated (bs-2067R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-PI3KCA Polyclonal Antibody, Unconjugated (bs-2067R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-PI3KCA Polyclonal Antibody, Unconjugated (bs-2067R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

PIK3CA Polyclonal Antibody

BS-2067R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPIK3CA
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    PI3KCA Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5290
  • Target name
    PIK3CA
  • Target description
    phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
  • Target synonyms
    CLAPO; CLOVE; CWS5; MCAP; MCM; MCMTC; mutant phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; p110-alpha; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphoinositide 3-kinase alpha; phosphoinositide-3-kinase, catalytic, alpha polypeptide; PI3K; PI3K-alpha; PI3-kinase p110 subunit alpha; ptdIns-3-kinase subunit p110-alpha; serine/threonine protein kinase PIK3CA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42336
  • Protein Name
    Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Ginsenoside Rb1 Prevents Dysfunction of Endothelial Cells by Suppressing Inflammatory Response and Apoptosis in the High-Fat Diet Plus Balloon Catheter-Injured Rabbit Model via the G Protein-Coupled Estrogen Receptor-Mediated Phosphatidylinositol 3-Kinases (PI3K)/Akt Pathway. Yang G et al., 2019 Oct 3, Med Sci Monit
    Read more
  • Lysine Enhances the Stimulation of Fatty Acids on Milk Fat Synthesis via the GPRC6A-PI3K-FABP5 Signaling in Bovine Mammary Epithelial Cells. Li X et al., 2019 Jun 26, J Agric Food Chem
    Read more
  • GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway. Li S et al., 2019 Nov, J Cell Physiol
    Read more
  • Deoxypodophyllotoxin inhibits cell viability and invasion by blocking the PI3K/Akt signaling pathway in human glioblastoma cells. Wang W et al., 2019 Apr, Oncol Rep
    Read more
  • Taurine Promotes Milk Synthesis via the GPR87-PI3K-SETD1A Signaling in BMECs. Yu M et al., 2019 Feb 20, J Agric Food Chem
    Read more
  • 8-Cetylcoptisine, a new coptisine derivative, induces mitochondria-dependent apoptosis and G0/G1 cell cycle arrest in human A549 cells. Han B et al., 2019 Feb 1, Chem Biol Interact
    Read more
  • Desumoylating Isopeptidase 2 (DESI2) Inhibits Proliferation and Promotes Apoptosis of Pancreatic Cancer Cells through Regulating PI3K/AKT/mTOR Signaling Pathway. Ou X et al., 2019 Apr, Pathol Oncol Res
    Read more
  • Methionine Promotes Milk Protein and Fat Synthesis and Cell Proliferation via the SNAT2-PI3K Signaling Pathway in Bovine Mammary Epithelial Cells. Qi H et al., 2018 Oct 24, J Agric Food Chem
    Read more

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