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Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-PI3KCA Polyclonal Antibody, Unconjugated (bs-2067R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-PI3KCA Polyclonal Antibody, Unconjugated (bs-2067R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-PI3KCA Polyclonal Antibody, Unconjugated (bs-2067R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

PIK3CA Polyclonal Antibody

BS-2067R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPIK3CA
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Overview

  • Supplier
    Bioss
  • Product Name
    PIK3CA Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5290
  • Target name
    PIK3CA
  • Target description
    phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
  • Target synonyms
    CCM4, CLAPO, CLOVE, CWS5, HMH, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha, phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3-kinase p110 subunit alpha, mutant phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha, phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha, phosphatidylinositol 3-kinase, catalytic, alpha polypeptide, phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, phosphoinositide 3-kinase alpha, phosphoinositide-3-kinase, catalytic, alpha polypeptide, ptdIns-3-kinase subunit p110-alpha, serine/threonine protein kinase PIK3CA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42336
  • Protein Name
    Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Yang G, Zhuo J, Lin Y, et al. Ginsenoside Rb1 Prevents Dysfunction of Endothelial Cells by Suppressing Inflammatory Response and Apoptosis in the High-Fat Diet Plus Balloon Catheter-Injured Rabbit Model via the G Protein-Coupled Estrogen Receptor-Mediated Phosphatidylinositol 3-Kinases (PI3K)/Akt Pathway. Med Sci Monit. 2019,25:7407-7417. doi: 10.12659/MSM.912986
    Read this paper
  • Li X, Li P, Wang L, et al. Lysine Enhances the Stimulation of Fatty Acids on Milk Fat Synthesis via the GPRC6A-PI3K-FABP5 Signaling in Bovine Mammary Epithelial Cells. J Agric Food Chem. 2019,67(25):7005-7015. doi: 10.1021/acs.jafc.9b02160
    Read this paper
  • Li S, Fu Y, Pang Y, et al. GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway. J Cell Physiol. 2019,234(11):21211-21223. doi: 10.1002/jcp.28727
    Read this paper
  • Wang W, Gao W, Zhang L, et al. Deoxypodophyllotoxin inhibits cell viability and invasion by blocking the PI3K/Akt signaling pathway in human glioblastoma cells. Oncol Rep. 2019,41(4):2453-2463. doi: 10.3892/or.2019.7016
    Read this paper
  • Yu M, Wang Y, Wang Z, et al. Taurine Promotes Milk Synthesis via the GPR87-PI3K-SETD1A Signaling in BMECs. J Agric Food Chem. 2019,67(7):1927-1936. doi: 10.1021/acs.jafc.8b06532
    Read this paper
  • Han B, Jiang P, Xu H, et al. 8-Cetylcoptisine, a new coptisine derivative, induces mitochondria-dependent apoptosis and G0/G1 cell cycle arrest in human A549 cells. Chem Biol Interact. 2019,299:27-36. doi: 10.1016/j.cbi.2018.11.005
    Read this paper
  • Ou X, Zhang GT, Xu Z, et al. Desumoylating Isopeptidase 2 (DESI2) Inhibits Proliferation and Promotes Apoptosis of Pancreatic Cancer Cells through Regulating PI3K/AKT/mTOR Signaling Pathway. Pathol Oncol Res. 2019,25(2):635-646. doi: 10.1007/s12253-018-0487-4
    Read this paper
  • Qi H, Meng C, Jin X, et al. Methionine Promotes Milk Protein and Fat Synthesis and Cell Proliferation via the SNAT2-PI3K Signaling Pathway in Bovine Mammary Epithelial Cells. J Agric Food Chem. 2018,66(42):11027-11033. doi: 10.1021/acs.jafc.8b04241
    Read this paper

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Figure 1. Western blot analysis of PIK3CA using anti-PIK3CA antibody (PB9314). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3CA antigen affinity purified polyclonal antibody (Catalog # PB9314) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3CA at approximately 124 kDa. The expected band size for PIK3CA is at 124 kDa.
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