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WB analysis of Jurkat cells using GTX45059 PI4KB antibody at 0.2-1microg/ml.
WB analysis of Jurkat cells using GTX45059 PI4KB antibody at 0.2-1microg/ml.
WB analysis of Jurkat cells using GTX45059 PI4KB antibody at 0.2-1microg/ml.

PI4 kinase beta antibody, N-term

GTX45059
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetPI4KB
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Overview

  • Supplier
    GeneTex
  • Product Name
    PI4 kinase beta antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5298
  • Target name
    PI4KB
  • Target description
    phosphatidylinositol 4-kinase beta
  • Target synonyms
    DFNA87, NPIK, PI4K-BETA, PI4K92, PI4KBETA, PI4KIII, PI4KIIIBETA, PIK4CB, phosphatidylinositol 4-kinase beta, PtdIns 4-kinase beta, phosphatidylinositol 4-kinase, catalytic, beta, phosphatidylinositol 4-kinase, wortmannin-sensitive, type III phosphatidylinositol 4-kinase beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBF8
  • Protein Name
    Phosphatidylinositol 4-kinase beta
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PI4KB using anti-PI4KB antibody (A04249-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI4KB antigen affinity purified polyclonal antibody (Catalog # A04249-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI4KB at approximately 95 kDa. The expected band size for PI4KB is at 91,91-110 kDa.
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