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Western Blot analysis of 293 cells using PIAS 3 Polyclonal Antibody
Western Blot analysis of 293 cells using PIAS 3 Polyclonal Antibody
Western Blot analysis of 293 cells using PIAS 3 Polyclonal Antibody

PIAS3 Antibody

CSB-PA003780
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPIAS3
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Overview

  • Supplier
    Cusabio
  • Product Name
    PIAS3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10401
  • Target name
    PIAS3
  • Target description
    protein inhibitor of activated STAT 3
  • Target synonyms
    E3 SUMO-protein ligase PIAS3; E3 SUMO-protein transferase PIAS3; protein inhibitor of activated STAT protein 3; zinc finger, MIZ-type containing 5; ZMIZ5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6X2
  • Protein Name
    E3 SUMO-protein ligase PIAS3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PIAS3 using anti-PIAS3 antibody (A02807-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate, Lane 2: human U-87MG cell lysate, Lane 3: human COLO-320 cell lysate, Lane 4: human HepG2 cell lysate, Lane 5: rat brain tissue lysate, Lane 6: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS3 antigen affinity purified polyclonal antibody (Catalog # A02807-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS3 at approximately 68KD. The expected band size for PIAS3 is at 68KD.
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