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IHC-P analysis of rat brain tissue using GTX31802 PIASy antibody. Working concentration : 5 microg/ml
IHC-P analysis of rat brain tissue using GTX31802 PIASy antibody. Working concentration : 5 microg/ml
IHC-P analysis of rat brain tissue using GTX31802 PIASy antibody. Working concentration : 5 microg/ml

PIASy antibody

GTX31802
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPIAS4
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Overview

  • Supplier
    GeneTex
  • Product Name
    PIASy antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 microg/mL. IHC-P: 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51588
  • Target name
    PIAS4
  • Target description
    protein inhibitor of activated STAT 4
  • Target synonyms
    PIAS-gamma, PIASY, Piasg, ZMIZ6, E3 SUMO-protein ligase PIAS4, RING-type E3 ubiquitin transferase PIAS4, protein inhibitor of activated STAT protein 4, protein inhibitor of activated STAT protein PIASy, protein inhibitor of activated STAT protein gamma, zinc finger, MIZ-type containing 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N2W9
  • Protein Name
    E3 SUMO-protein ligase PIAS4
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of PIAS4 using anti-PIAS4 antibody (PB10082). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS4 antigen affinity purified polyclonal antibody (Catalog # PB10082) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS4 at approximately 57 kDa. The expected band size for PIAS4 is at 57 kDa.
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