Pig ASMA(Anti-Smooth Muscle Antibody) ELISA Kit

Biorbyt

Pig ASMA(Anti-Smooth Muscle Antibody) ELISA Kit

10

standard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig ASMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ASMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig ASMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pig ASMA in the samples is then determined by comparing the OD of the samples to the standard curve

7.82-500 ng/mL

2.8 ng/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anti-Smooth Muscle Antibody(ASMA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Smooth Muscle Antibody(ASMA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Smooth Muscle Antibody(ASMA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Smooth Muscle Antibody(ASMA) in the samples is then determined by comparing the OD of the samples to the standard curve.

41116158