Pig VIM(Vimentin) ELISA Kit

Biorbyt

Pig VIM(Vimentin) ELISA Kit

10

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig VIM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig VIM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig VIM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pig VIM in the samples is then determined by comparing the OD of the samples to the standard curve

1.57-100 ng/mL

0.61 ng/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vimentin(VIM). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vimentin(VIM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vimentin(VIM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Vimentin(VIM) in the samples is then determined by comparing the OD of the samples to the standard curve.

41116158