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Western Blot Positive WB detected in: A549 whole cell lysate All lanes: PIGV antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa
Western Blot Positive WB detected in: A549 whole cell lysate All lanes: PIGV antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa
Western Blot Positive WB detected in: A549 whole cell lysate All lanes: PIGV antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa

PIGV Antibody

CSB-PA868321LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPIGV
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Overview

  • Supplier
    Cusabio
  • Product Name
    PIGV Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55650
  • Target name
    PIGV
  • Target description
    phosphatidylinositol glycan anchor biosynthesis class V
  • Target synonyms
    GPI-MT-II, HPMRS1, PIG-V, GPI alpha-1,6-mannosyltransferase 2, GPI mannosyltransferase 2, GPI mannosyltransferase II, Ybr004c homolog, dol-P-Man dependent GPI mannosyltransferase, dol-P-Man dependent GPI mannosyltransferase II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NUD9
  • Protein Name
    GPI alpha-1,6-mannosyltransferase 2
  • Scientific Description
    Alpha-1,6-mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the second mannose to the glycosylphosphatidylinositol during GPI precursor assembly.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PIGV using anti-PIGV antibody (A08698-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIGV antigen affinity purified polyclonal antibody (Catalog # A08698-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIGV at approximately 60 kDa. The expected band size for PIGV is at 56 kDa.
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