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PIK3CA Rabbit pAb

A0265
ABclonal
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPIK3CA
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Overview

  • Supplier
    ABclonal
  • Product Name
    PIK3CA Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5290
  • Target name
    PIK3CA
  • Target description
    phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
  • Target synonyms
    CCM4, CLAPO, CLOVE, CWS5, HMH, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha, phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3-kinase p110 subunit alpha, mutant phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha, phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha, phosphatidylinositol 3-kinase, catalytic, alpha polypeptide, phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, phosphoinositide 3-kinase alpha, phosphoinositide-3-kinase, catalytic, alpha polypeptide, ptdIns-3-kinase subunit p110-alpha, serine/threonine protein kinase PIK3CA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42336
  • Protein Name
    Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PIK3CA using anti-PIK3CA antibody (PB9314). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3CA antigen affinity purified polyclonal antibody (Catalog # PB9314) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3CA at approximately 124 kDa. The expected band size for PIK3CA is at 124 kDa.
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