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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate All lanes: PIK3R6 antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 85 kDa Observed band size: 85 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate All lanes: PIK3R6 antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 85 kDa Observed band size: 85 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate All lanes: PIK3R6 antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 85 kDa Observed band size: 85 kDa

PIK3R6 Antibody

CSB-PA719469LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPIK3R6
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Overview

  • Supplier
    Cusabio
  • Product Name
    PIK3R6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID146850
  • Target name
    PIK3R6
  • Target description
    phosphoinositide-3-kinase regulatory subunit 6
  • Target synonyms
    C17orf38, HsT41028, p84 PIKAP, p87(PIKAP), p87PIKAP, phosphoinositide 3-kinase regulatory subunit 6, PI3Kgamma adapter protein of 87 kDa, p84 PI3K adapter protein, p87 phosphoinositide 3-kinase gamma (PI3Kg) adapter protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5UE93
  • Protein Name
    Phosphoinositide 3-kinase regulatory subunit 6
  • Scientific Description
    Regulatory subunit of the PI3K gamma complex. Acts as an adapter to drive activation of PIK3CG by beta-gamma G protein dimers. The PIK3CG:PIK3R6 heterodimer is much less sensitive to beta-gamma G protein dimers than PIK3CG:PIK3R5 and its membrane recruitment and beta-gamma G protein dimer-dependent activation requires HRAS bound to PIK3CG. Recruits of the PI3K gamma complex to a PDE3B:RAPGEF3 signaling complex involved in angiogenesis; signaling seems to involve RRAS.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PIK3R6 using anti-PIK3R6 antibody (A14338-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R6 antigen affinity purified polyclonal antibody (Catalog # A14338-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3R6 at approximately 84 kDa. The expected band size for PIK3R6 is at 84 kDa.
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