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ProductsBack/PILRA Antibody (FITC)

PILRA Antibody (FITC)

LS-C398275
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetPILRA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PILRA Antibody (FITC)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.25 mg/ml
  • Conjugate
    FITC
  • Estimated Purity
    ...
  • Gene ID29992
  • Target name
    PILRA
  • Target description
    paired immunoglobin like type 2 receptor alpha
  • Target synonyms
    FDF03, paired immunoglobulin-like type 2 receptor alpha, cell surface receptor FDF03, inhibitory receptor PILR-alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PILRA using anti-PILRA antibody (A07502-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PILRA antigen affinity purified polyclonal antibody (Catalog # A07502-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PILRA at approximately 61 kDa. The expected band size for PILRA is at 34 kDa.
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