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ProductsBack/PINK1 antibody [N3C3]

PINK1 antibody [N3C3]

GTX107851
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetPINK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PINK1 antibody [N3C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.63 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID65018
  • Target name
    PINK1
  • Target description
    PTEN induced kinase 1
  • Target synonyms
    BRPK; PARK6; protein kinase BRPK; PTEN induced putative kinase 1; PTEN-induced putative kinase protein 1; serine/threonine-protein kinase PINK1, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BXM7
  • Protein Name
    Serine/threonine-protein kinase PINK1, mitochondrial
  • Scientific Description
    This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Therapeutic effects of the combination of moderate-intensity endurance training and MitoQ supplementation in rats with isoproterenol-induced myocardial injury: The role of mitochondrial fusion, fission, and mitophagy.
    Read more
  • Abnormalities in the SIRT1-SIRT3 axis promote myocardial ischemia-reperfusion injury through ferroptosis caused by silencing the PINK1/Parkin signaling pathway.
    Read more
  • Parkin, as a Regulator, Participates in Arsenic Trioxide-Triggered Mitophagy in HeLa Cells. Zhang Z et al., 2022 Jun 20, Curr Issues Mol Biol
    Read more
  • The nuclear receptor RORalpha preserves cardiomyocyte mitochondrial function by regulating caveolin-3-mediated mitophagy. Beak JY et al., 2021 Dec, J Biol Chem
    Read more
  • Cardiac-specific microRNA-125b deficiency induces perinatal death and cardiac hypertrophy. Chen CY et al., 2021 Jan 27, Sci Rep
    Read more
  • Mitochondrial Metabolism as Target of the Neuroprotective Role of Erythropoietin in Parkinsons Disease. Rey F et al., 2021 Jan 15, Antioxidants (Basel)
    Read more
  • The potential LXRbeta agonist stigmasterol protects against hypoxia/reoxygenation injury by modulating mitophagy in primary hippocampal neurons. Haque MN et al., 2021 Jan, Phytomedicine
    Read more
  • Downregulation of Mitochondrial TSPO Inhibits Mitophagy and Reduces Enucleation during Human Terminal Erythropoiesis. Moras M et al., 2020 Nov 28, Int J Mol Sci
    Read more
  • Astragaloside IV ameliorates diabetic nephropathy by modulating the mitochondrial quality control network. Liu X et al., 2017, PLoS One
    Read more
  • NBR1 is dispensable for PARK2-mediated mitophagy regardless of the presence or absence of SQSTM1. Shi J et al., 2015 Oct 29, Cell Death Dis
    Read more

Datasheet

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Figure 1. Western blot analysis of PINK1 using anti-PINK1 antibody (A00201-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PINK1antigen affinity purified polyclonal antibody (Catalog # A00201-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PINK1 at approximately 64KD. The expected band size for PINK1 is at 64KD.
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