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PINX1 Antibody

LS-C501513
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPINX1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PINX1 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54984
  • Target name
    PINX1
  • Target description
    PIN2 (TERF1) interacting telomerase inhibitor 1
  • Target synonyms
    Gno1, LPTL, LPTS, Pxr1, PIN2/TERF1-interacting telomerase inhibitor 1, 67-11-3 protein, PIN2-interacting protein 1, TRF1-interacting protein 1, hepatocellular carcinoma-related putative tumor suppressor, liver-related putative tumor suppressor, pin2-interacting protein X1, protein 67-11-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PINX1 using anti-PINX1 antibody (PB9498). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: HUT Whole Cell Lysate at 40ug, Lane 4: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PINX1 antigen affinity purified polyclonal antibody (Catalog # PB9498) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PINX1 at approximately 37 kDa. The expected band size for PINX1 is at 37 kDa.
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