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Western Blot analysis of 3T3 cells using PIPOX Polyclonal Antibody
Western Blot analysis of 3T3 cells using PIPOX Polyclonal Antibody
Western Blot analysis of 3T3 cells using PIPOX Polyclonal Antibody

PIPOX Antibody

CSB-PA020276
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPIPOX
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Overview

  • Supplier
    Cusabio
  • Product Name
    PIPOX Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51268
  • Target name
    PIPOX
  • Target description
    pipecolic acid and sarcosine oxidase
  • Target synonyms
    L-pipecolate oxidase; L-pipecolic acid oxidase; LPIPOX; peroxisomal sarcosine oxidase; pipecolic acid oxidase; PSO
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P0Z9
  • Protein Name
    Peroxisomal sarcosine oxidase
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PIPOX using anti-PIPOX antibody (A10475-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIPOX antigen affinity purified polyclonal antibody (Catalog # A10475-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIPOX at approximately 40 kDa. The expected band size for PIPOX is at 44 kDa.
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WB analysis of NIH/3T3 and RAW264.7 cell lysates using GTX87135 PIPOX antibody. The lane on the right is blocked with the synthesized peptide.
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