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Western Blot analysis of HepG2 cells using PITPbeta Polyclonal Antibody
Western Blot analysis of HepG2 cells using PITPbeta Polyclonal Antibody
Western Blot analysis of HepG2 cells using PITPbeta Polyclonal Antibody

PITPNB Antibody

CSB-PA003789
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPITPNB
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Overview

  • Supplier
    Cusabio
  • Product Name
    PITPNB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23760
  • Target name
    PITPNB
  • Target description
    phosphatidylinositol transfer protein beta
  • Target synonyms
    PI-TP-beta, PtdInsTP, VIB1B, phosphatidylinositol transfer protein beta isoform, PtdIns transfer protein beta, phosphotidylinositol transfer protein, beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48739
  • Protein Name
    Phosphatidylinositol transfer protein beta isoform
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PITPNB using anti-PITPNB antibody (A11315-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tisue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PITPNB antigen affinity purified polyclonal antibody (Catalog # A11315-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PITPNB at approximately 35 kDa. The expected band size for PITPNB is at 32,35 kDa.
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