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Western blot All lanes: PITRM1 antibody at 2microg/ml Lane 1: A549 whole cell lysate Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 118, 107 kDa Observed band size: 118 kDa
Western blot All lanes: PITRM1 antibody at 2microg/ml Lane 1: A549 whole cell lysate Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 118, 107 kDa Observed band size: 118 kDa
Western blot All lanes: PITRM1 antibody at 2microg/ml Lane 1: A549 whole cell lysate Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 118, 107 kDa Observed band size: 118 kDa

PITRM1 Antibody

CSB-PA692283ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPITRM1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PITRM1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10531
  • Target name
    PITRM1
  • Target description
    pitrilysin metallopeptidase 1
  • Target synonyms
    metalloprotease 1 (pitrilysin family); MP1; pitrilysin metalloproteinase 1; PreP; PreP peptidasome; presequence protease, mitochondrial; SCAR30
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5JRX3
  • Protein Name
    Presequence protease, mitochondrial
  • Scientific Description
    ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PITRM1 using anti-PITRM1 antibody (A08969-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PITRM1 antigen affinity purified polyclonal antibody (Catalog # A08969-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PITRM1 at approximately 117 kDa. The expected band size for PITRM1 is at 117 kDa.
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