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Western blot analysis of extracts of various cell lines using PIWIL4 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using PIWIL4 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using PIWIL4 Polyclonal Antibody at dilution of 1:3000.

PIWIL4 Polyclonal Antibody

E-AB-64500
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    PIWIL4 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells. Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PIWIL4/PIWI using anti-PIWIL4/PIWI antibody (A06797-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIWIL4/PIWI antigen affinity purified polyclonal antibody (Catalog # A06797-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIWIL4/PIWI at approximately 97 kDa. The expected band size for PIWIL4/PIWI is at 97 kDa.
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