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PKC gamma Rabbit mAb

A9565
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRKCG
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Overview

  • Supplier
    ABclonal
  • Product Name
    PKC gamma Rabbit mAb
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID5582
  • Target name
    PRKCG
  • Target description
    protein kinase C gamma
  • Target synonyms
    PKC-gamma, PKCC, PKCG, PKCI(3), PKCgamma, SCA14, protein kinase C gamma type
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05129
  • Protein Name
    Protein kinase C gamma type
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PKC gamma using anti-PKC gamma antibody (A01890). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC gamma antigen affinity purified polyclonal antibody (Catalog # A01890) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC gamma at approximately 78 kDa. The expected band size for PKC gamma is at 78 kDa.
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