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ProductsBack/PKC iota / lambda (phospho Thr563) antibody
WB (peptide competition) analysis of Jurkat cells stimulated with PMA using GTX25813 PKC iota / lambda (phospho Thr563) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
WB (peptide competition) analysis of Jurkat cells stimulated with PMA using GTX25813 PKC iota / lambda (phospho Thr563) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
WB (peptide competition) analysis of Jurkat cells stimulated with PMA using GTX25813 PKC iota / lambda (phospho Thr563) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

PKC iota / lambda (phospho Thr563) antibody

GTX25813
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
ReactivityCanine, Human, Mouse
TargetPRKCI
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Overview

  • Supplier
    GeneTex
  • Product Name
    PKC iota / lambda (phospho Thr563) antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Antibody Specificity
    This antibody reacts with PKC lambda immunoprecipitates, indicating cross-reactivity for PKC lambda [pT563]. PKC zeta [pT56] (83% homologous) has been shown to cross-react by peptide competition. Peptide competition also suggests that this antibody may pa
  • Application Supplier Note
    FACS: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5584
  • Target name
    PRKCI
  • Target description
    protein kinase C iota
  • Target synonyms
    aPKC-lambda/iota; atypical protein kinase C-lambda/iota; DXS1179E; nPKC-iota; PKCI; PRKC-lambda/iota; protein kinase C iota type
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41743
  • Protein Name
    Protein kinase C iota type
  • Scientific Description
    This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]
  • Reactivity
    Canine, Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID).
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  • Conditional knockout of polarity complex (atypical) PKCiota reveals an anti-inflammatory function mediated by NF-kappaB. Forteza R et al., 2016 Jul 15, Mol Biol Cell
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  • Myosin 5b loss of function leads to defects in polarized signaling: implication for microvillus inclusion disease pathogenesis and treatment. Kravtsov D et al., 2014 Nov 15, Am J Physiol Gastrointest Liver Physiol
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  • The BAG-1 isoform BAG-1M regulates keratin-associated Hsp70 chaperoning of aPKC in intestinal cells during activation of inflammatory signaling. Mashukova A et al., 2014 Aug 15, J Cell Sci
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  • Par-complex aPKC and Par3 cross-talk with innate immunity NF-kappaB pathway in epithelial cells. Forteza R et al., 2013, Biol Open
    Read more