PKD2 antibody [C1C3] detects PKD2 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PKD2 antibody [C1C3] (GTX102212) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![PKD2 antibody [C1C3] detects PKD2 protein at cytosol and membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. PKD2 antibody [C1C3] (GTX102212) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102212/GTX102212_41066_IHC_w_23060100_599.webp "PKD2 antibody [C1C3] detects PKD2 protein at cytosol and membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. PKD2 antibody [C1C3] (GTX102212) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![PKD2 antibody [C1C3] detects PKD2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PKD2 protein stained by PKD2 antibody [C1C3] (GTX102212) diluted at 1:200. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102212/GTX102212_41066_IFA_w_23060100_348.webp "PKD2 antibody [C1C3] detects PKD2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PKD2 protein stained by PKD2 antibody [C1C3] (GTX102212) diluted at 1:200. Blue: Hoechst 33342 staining.")
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKD2 antibody [C1C3] (GTX102212) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102212/GTX102212_41066_20160505_WB_w_23060100_139.webp "Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKD2 antibody [C1C3] (GTX102212) diluted at 1:500.")
PKD2 antibody [C1C3] detects PKD2 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PKD2 antibody [C1C3] (GTX102212) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
PKD2 antibody [C1C3]
GTX102212
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetPRKD2
Overview
- SupplierGeneTex
- Product NamePKD2 antibody [C1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.99 mg/ml
- ConjugateUnconjugated
- Gene ID25865
- Target namePRKD2
- Target descriptionprotein kinase D2
- Target synonymsHSPC187, PKD2, nPKC-D2, serine/threonine-protein kinase D2
- HostRabbit
- IsotypeIgG
- Protein IDQ9BZL6
- Protein NameSerine/threonine-protein kinase D2
- Scientific DescriptionThe protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Tandon M, Salamoun JM, Carder EJ, et al. SD-208, a novel protein kinase D inhibitor, blocks prostate cancer cell proliferation and tumor growth in vivo by inducing G2/M cell cycle arrest. PLoS One. 2015,10(3):e0119346. doi: 10.1371/journal.pone.0119346Read this paper
Datasheet
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