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ProductsBack/PKM2 (PKM) (N-term) Rabbit Polyclonal Antibody
Western blot analysis of PKM2 (arrow) using PKM2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKM2 gene (Lane 2).
Western blot analysis of PKM2 (arrow) using PKM2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKM2 gene (Lane 2).
Western blot analysis of PKM2 (arrow) using PKM2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKM2 gene (Lane 2).

PKM2 (PKM) (N-term) Rabbit Polyclonal Antibody

AP53318PU-N
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPKM
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    PKM2 (PKM) (N-term) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5315
  • Target name
    PKM
  • Target description
    pyruvate kinase M1/2
  • Target synonyms
    CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, p58, pyruvate kinase PKM, OPA-interacting protein 3, PK, muscle type, cytosolic thyroid hormone-binding protein, epididymis secretory protein Li 30, pyruvate kinase 2/3, pyruvate kinase isozymes M1/M2, pyruvate kinase muscle isozyme, pyruvate kinase, muscle, threonine-protein kinase PKM2, thyroid hormone-binding protein 1, thyroid hormone-binding protein, cytosolic, tumor M2-PK, tyrosine-protein kinase PKM2
  • Host
    Rabbit
  • Protein IDP14618
  • Protein Name
    Pyruvate kinase PKM
  • Scientific Description
    PKM2 (PKM) (N-term) rabbit polyclonal antibody, Purified
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PKM2 using anti-PKM2 antibody (PB9379). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human MDA-B-453 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat L6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKM2 antigen affinity purified polyclonal antibody (Catalog # PB9379) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKM2 at approximately 58 kDa. The expected band size for PKM2 is at 58 kDa.
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