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Mouse heart lysates probed with PKM2 Polyclonal Antibody, unconjugated (bs-0101R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Mouse heart lysates probed with PKM2 Polyclonal Antibody, unconjugated (bs-0101R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Mouse heart lysates probed with PKM2 Polyclonal Antibody, unconjugated (bs-0101R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

PKM2 Polyclonal Antibody

BS-0101R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPKLR
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Overview

  • Supplier
    Bioss
  • Product Name
    PKM2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5313
  • Target name
    PKLR
  • Target description
    pyruvate kinase L/R
  • Target synonyms
    CNSHA2, PK1, PKL, PKRL, RPK, pyruvate kinase PKLR, R-type/L-type pyruvate kinase, pyruvate kinase 1, pyruvate kinase isozyme R/L, pyruvate kinase isozymes L/R, pyruvate kinase isozymes R/L, pyruvate kinase type L, pyruvate kinase, liver and RBC, pyruvate kinase, liver and blood cell, red cell/liver pyruvate kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14618
  • Protein Name
    Pyruvate kinase PKM
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of PKLR using anti-PKLR antibody (PB9499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKLR antigen affinity purified polyclonal antibody (Catalog # PB9499) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKLR at approximately 62 kDa. The expected band size for PKLR is at 62 kDa.
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Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum & the Golgi apparatus.
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