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WB analysis of HeLa cell lysate using GTX25440 PKN beta antibody.
WB analysis of HeLa cell lysate using GTX25440 PKN beta antibody.
WB analysis of HeLa cell lysate using GTX25440 PKN beta antibody.

PKN beta antibody

GTX25440
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPKN3
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Overview

  • Supplier
    GeneTex
  • Product Name
    PKN beta antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. IHC-P: 1:10-1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29941
  • Target name
    PKN3
  • Target description
    protein kinase N3
  • Target synonyms
    protein kinase PKN-beta; protein-kinase C-related kinase 3; serine/threonine-protein kinase N3; UTDP4-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6P5Z2
  • Protein Name
    Serine/threonine-protein kinase N3
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PKN3 using anti-PKN3 antibody (A09775-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKN3 antigen affinity purified polyclonal antibody (Catalog # A09775-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKN3 at approximately 99 kDa. The expected band size for PKN3 is at 99 kDa.
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