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PLA2G15 / ACS Antibody

LS-C830969
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPLA2G15
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PLA2G15 / ACS Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:50 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23659
  • Target name
    PLA2G15
  • Target description
    phospholipase A2 group XV
  • Target synonyms
    ACS, GXVPLA2, LLPL, LPLA2, LYPLA3, lysosomal phospholipase A and acyltransferase, 1-O-acylceramide synthase, BMP hydrolase, LCAT-like lysophospholipase, group XV phospholipase A2, lysophospholipase 3 (lysosomal phospholipase A2), lysosomal phospholipase A2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human colon, kidney, lymph node and placenta using Anti-PLA2G15 antibody HPA041702 (A) shows similar protein distribution across tissues to independent antibody HPA041727 (B).
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Figure 1. Western blot analysis of LYPLA3/PLA2G15 using anti-LYPLA3/PLA2G15 antibody (A08649). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYPLA3/PLA2G15 antigen affinity purified polyclonal antibody (Catalog # A08649) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYPLA3/PLA2G15 at approximately 42-57 kDa. The expected band size for LYPLA3/PLA2G15 is at 65-70 kDa.
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