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ProductsBack/PLA2G4B Antibody
Western Blot Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate All lanes: P0C869 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 88, 101, 55, 115 kDa Observed band size: 88 kDa
Western Blot Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate All lanes: P0C869 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 88, 101, 55, 115 kDa Observed band size: 88 kDa
Western Blot Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate All lanes: P0C869 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 88, 101, 55, 115 kDa Observed band size: 88 kDa

PLA2G4B Antibody

CSB-PA018098LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetJMJD7-PLA2G4B
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Overview

  • Supplier
    Cusabio
  • Product Name
    PLA2G4B Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8681
  • Target name
    JMJD7-PLA2G4B
  • Target description
    JMJD7-PLA2G4B readthrough
  • Target synonyms
    HsT16992, cPLA2-beta, JMJD7-PLA2G4B protein, jumonji domain containing 7-phospholipase A2, group IVB (cytosolic) read-through
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP0C869
  • Protein Name
    Cytosolic phospholipase A2 beta
  • Scientific Description
    Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. Has a much weaker activity than PLA2G4A. Isoform 3 has calcium-dependent activity against palmitoyl-arachidonyl-phosphatidylethanolamine and low level lysophospholipase activity but no activity against phosphatidylcholine. Isoform 5 does have activity against phosphatidylcholine.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of cPLA2 beta/PLA2G4B using anti-cPLA2 beta/PLA2G4B antibody (A11137-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cPLA2 beta/PLA2G4B antigen affinity purified polyclonal antibody (Catalog # A11137-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for cPLA2 beta/PLA2G4B at approximately 88KD. The expected band size for cPLA2 beta/PLA2G4B is at 88KD.
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