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Plasmid / vector

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Genomic editing of RFP deletion was performed using wild type RFP sequence (oligo donor), and verified gRNA plasmid. The gRNA sequence was designed based on NGG PAM sequence, and cloned in the xCas9 (Cat#GE100078) or SpCas9 (Cat#GE100002) vector. The parent cell used for the gene editing contains a RFP expression cassette which has a big deletion in the coding sequence. 0.5ug of gRNA plasmid and 0.5ug RFP repairing oligo were co-transfected in the RFP negative parent cells. 10 days after transfection, RFP fluorescence can be observed (Left panel) in both xCas9 and SpCas9 transfected cells. However, xCas9 (Top) seems to have a higher editing efficiency than SpCas9 (bottom).
Product group DNA / RNA / Vectors
OriGene
RFP negative transgenic cell lines (HEK293T cells containing RFP dead mutant at AAVS1 locus)GE100041C
  • SizePrice
Genomic editing of RFP deletion was performed using wild type RFP sequence (oligo donor), and verified gRNA plasmid. The gRNA sequence was designed based on NGG PAM sequence, and cloned in the xCas9 (Cat#GE100078) or SpCas9 (Cat#GE100002) vector. The parent cell used for the gene editing contains a RFP expression cassette which has a big deletion in the coding sequence. 0.5ug of gRNA plasmid and 0.5ug RFP repairing oligo were co-transfected in the RFP negative parent cells. 10 days after transfection, RFP fluorescence can be observed (Left panel) in both xCas9 and SpCas9 transfected cells. However, xCas9 (Top) seems to have a higher editing efficiency than SpCas9 (bottom).
Product group DNA / RNA / Vectors
OriGene
Validated repairing donor oligos for RFP dead mutant correction in GE100041C cellsGE100041D
  • SizePrice
Genomic editing of RFP deletion was performed using wild type RFP sequence (oligo donor), and verified gRNA plasmid. The gRNA sequence was designed based on NGG PAM sequence, and cloned in the xCas9 (Cat#GE100078) or SpCas9 (Cat#GE100002) vector. The parent cell used for the gene editing contains a RFP expression cassette which has a big deletion in the coding sequence. 0.5ug of gRNA plasmid and 0.5ug RFP repairing oligo were co-transfected in the RFP negative parent cells. 10 days after transfection, RFP fluorescence can be observed (Left panel) in both xCas9 and SpCas9 transfected cells. However, xCas9 (Top) seems to have a higher editing efficiency than SpCas9 (bottom).
Product group DNA / RNA / Vectors
OriGene
Validated sgRNA targeting RFP dead mutant in GE100041C cellsGE100041G
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pGuide
Product group DNA / RNA / Vectors
OriGene
pGuideGE100042
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pGuide-EF1a-GFP
Product group DNA / RNA / Vectors
OriGene
pGuide-EF1a-GFPGE100044
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pLenti-EF1a-Cas-Guide
Product group DNA / RNA / Vectors
OriGene
pLenti-EF1a-Cas-GuideGE100045
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pAAVS1-EF1a-puro-DNR CRISPR Vector
Product group DNA / RNA / Vectors
OriGene
pAAVS1-EF1a-puro-DNR CRISPR VectorGE100046
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AAVS1 Transgene Knockin kit (EF1a, Puro)
Product group DNA / RNA / Vectors
OriGene
AAVS1 Transgene Knockin kit (EF1a, Puro)GE100047
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pAAVS1-EF1a-BSD-DNR
Product group DNA / RNA / Vectors
OriGene
pAAVS1-EF1a-BSD-DNRGE100048
  • SizePrice
AAVS1 Transgene Knockin kit (EF1a, BSD)
Product group DNA / RNA / Vectors
OriGene
AAVS1 Transgene Knockin kit (EF1a, BSD)GE100049
  • SizePrice
pCas-Guide-ROSA26
Product group DNA / RNA / Vectors
OriGene
pCas-Guide-ROSA26GE100050
  • SizePrice
pROSA26-Puro-DNR
Product group DNA / RNA / Vectors
OriGene
pROSA26-Puro-DNRGE100051
  • SizePrice
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