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Plasmid / vector

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pLenti-EF1a-Cas9-IRES-Puro
Product group DNA / RNA / Vectors
OriGene
pLenti-EF1a-Cas9-IRES-PuroGE100030
CategoryPlasmid / vector
  • SizePrice
pLenti-Cas9-P2A-tGFP
Product group DNA / RNA / Vectors
OriGene
pLenti-Cas9-P2A-tGFPGE100031
CategoryPlasmid / vector
  • SizePrice
pLenti-Guide-Puro
Product group DNA / RNA / Vectors
OriGene
pLenti-Guide-PuroGE100032
CategoryPlasmid / vector
  • SizePrice
pAAVS1-Cas9-Puro-DNR
Product group DNA / RNA / Vectors
OriGene
pAAVS1-Cas9-Puro-DNRGE100037
CategoryPlasmid / vector
  • SizePrice
Cas9 insertion kit at AAVS1 locus (Puro selection)
Product group DNA / RNA / Vectors
OriGene
Cas9 insertion kit at AAVS1 locus (Puro selection)GE100038
CategoryPlasmid / vector
  • SizePrice
pAAVS1-Cas9-BSD-DNR
Product group DNA / RNA / Vectors
OriGene
pAAVS1-Cas9-BSD-DNRGE100039
CategoryPlasmid / vector
  • SizePrice
Cas9 insertion kit at AAVS1 locus (BSD selection)
Product group DNA / RNA / Vectors
OriGene
Cas9 insertion kit at AAVS1 locus (BSD selection)GE100040
CategoryPlasmid / vector
  • SizePrice
Genomic editing of RFP deletion was performed using wild type RFP sequence (oligo donor), and verified gRNA plasmid. The gRNA sequence was designed based on NGG PAM sequence, and cloned in the xCas9 (Cat#GE100078) or SpCas9 (Cat#GE100002) vector. The parent cell used for the gene editing contains a RFP expression cassette which has a big deletion in the coding sequence. 0.5ug of gRNA plasmid and 0.5ug RFP repairing oligo were co-transfected in the RFP negative parent cells. 10 days after transfection, RFP fluorescence can be observed (Left panel) in both xCas9 and SpCas9 transfected cells. However, xCas9 (Top) seems to have a higher editing efficiency than SpCas9 (bottom).
Product group DNA / RNA / Vectors
OriGene
RFP negative transgenic cell lines (HEK293T cells containing RFP dead mutant at AAVS1 locus)GE100041C
CategoryPlasmid / vector
  • SizePrice
Genomic editing of RFP deletion was performed using wild type RFP sequence (oligo donor), and verified gRNA plasmid. The gRNA sequence was designed based on NGG PAM sequence, and cloned in the xCas9 (Cat#GE100078) or SpCas9 (Cat#GE100002) vector. The parent cell used for the gene editing contains a RFP expression cassette which has a big deletion in the coding sequence. 0.5ug of gRNA plasmid and 0.5ug RFP repairing oligo were co-transfected in the RFP negative parent cells. 10 days after transfection, RFP fluorescence can be observed (Left panel) in both xCas9 and SpCas9 transfected cells. However, xCas9 (Top) seems to have a higher editing efficiency than SpCas9 (bottom).
Product group DNA / RNA / Vectors
OriGene
Validated repairing donor oligos for RFP dead mutant correction in GE100041C cellsGE100041D
CategoryPlasmid / vector
  • SizePrice
Genomic editing of RFP deletion was performed using wild type RFP sequence (oligo donor), and verified gRNA plasmid. The gRNA sequence was designed based on NGG PAM sequence, and cloned in the xCas9 (Cat#GE100078) or SpCas9 (Cat#GE100002) vector. The parent cell used for the gene editing contains a RFP expression cassette which has a big deletion in the coding sequence. 0.5ug of gRNA plasmid and 0.5ug RFP repairing oligo were co-transfected in the RFP negative parent cells. 10 days after transfection, RFP fluorescence can be observed (Left panel) in both xCas9 and SpCas9 transfected cells. However, xCas9 (Top) seems to have a higher editing efficiency than SpCas9 (bottom).
Product group DNA / RNA / Vectors
OriGene
Validated sgRNA targeting RFP dead mutant in GE100041C cellsGE100041G
CategoryPlasmid / vector
  • SizePrice
pGuide
Product group DNA / RNA / Vectors
OriGene
pGuideGE100042
CategoryPlasmid / vector
  • SizePrice
pGuide-EF1a-GFP
Product group DNA / RNA / Vectors
OriGene
pGuide-EF1a-GFPGE100044
CategoryPlasmid / vector
  • SizePrice
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