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ProductsBack/Plastin L (LCP1) Rabbit Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using LCP1 antibody.
Western blot analysis of extracts of various cell lines, using LCP1 antibody.
Western blot analysis of extracts of various cell lines, using LCP1 antibody.

Plastin L (LCP1) Rabbit Polyclonal Antibody

TA332766
OriGene
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetLCP1
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Plastin L (LCP1) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID3936
  • Target name
    LCP1
  • Target description
    lymphocyte cytosolic protein 1
  • Target synonyms
    CP64, HEL-S-37, L-PLASTIN, LC64P, LPL, PLS2, plastin-2, L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P), LCP-1, Lymphocyte cytosolic protein-1 (plasmin), bA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin)), epididymis secretory protein Li 37
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13796
  • Protein Name
    Plastin-2
  • Scientific Description
    Rabbit anti-LCP1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of LCP1 using anti-LCP1 antibody (A03361). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCP1 antigen affinity purified polyclonal antibody (Catalog # A03361) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCP1 at approximately 70 kDa. The expected band size for LCP1 is at 70 kDa.
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