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Plaur Polyclonal Antibody

CAC08728
Biomatik
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPlaur
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Overview

  • Supplier
    Biomatik
  • Product Name
    Plaur Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB; Recommended dilution: WB:1:1000-1:5000
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID50692
  • Target name
    Plaur
  • Target description
    plasminogen activator, urokinase receptor
  • Target synonyms
    Par, Plaur3, uPAR, uPAR-2, uPAR-3, urokinase plasminogen activator surface receptor, U-PAR, plasminogen activator urokinase receptor 3, urinary plasminogen activator receptor 2, urinary plasminogen activator receptor 3, urokinase plasminogen activator receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49616
  • Protein Name
    Urokinase plasminogen activator surface receptor
  • Scientific Description
    The Plaur Polyclonal Antibody (Species: Rat) has been validated for the following applications: ELISA, WB.
  • Reactivity Supplier
    Rat, Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of uPA Receptor using anti-uPA Receptor antibody (A00993-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: rat small intestine tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse pancreas tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-uPA Receptor antigen affinity purified polyclonal antibody (Catalog # A00993-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for uPA Receptor at approximately 37KD. The expected band size for uPA Receptor is at 37KD.
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