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ProductsBack/PLCG 2 (PLCG2) Rabbit Polyclonal Antibody
Western blot analysis of extracts of Raji cells, using Phospho-PLC gamma 2 (PLCG2)-Y1217 antibody (TA380021) at 1:1000 dilution or PLC gamma 2 (PLCG2) antibody (A2182). Raji cells were treated by Pervanadate (1 mM) at 37°C for 30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 1s.
Western blot analysis of extracts of Raji cells, using Phospho-PLC gamma 2 (PLCG2)-Y1217 antibody (TA380021) at 1:1000 dilution or PLC gamma 2 (PLCG2) antibody (A2182). Raji cells were treated by Pervanadate (1 mM) at 37°C for 30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 1s.
Western blot analysis of extracts of Raji cells, using Phospho-PLC gamma 2 (PLCG2)-Y1217 antibody (TA380021) at 1:1000 dilution or PLC gamma 2 (PLCG2) antibody (A2182). Raji cells were treated by Pervanadate (1 mM) at 37°C for 30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 1s.

PLCG 2 (PLCG2) Rabbit Polyclonal Antibody

TA380021
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPLCG2
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    PLCG 2 (PLCG2) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5336
  • Target name
    PLCG2
  • Target description
    phospholipase C gamma 2
  • Target synonyms
    APLAID, FCAS3, PLC-IV, PLC-gamma-2, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2, phosphoinositide phospholipase C-gamma-2, phospholipase C, gamma 2 (phosphatidylinositol-specific), phospholipase C-IV
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16885
  • Protein Name
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
  • Scientific Description
    Phospho-PLC gamma 2 (PLCG2)-Y1217 Rabbit polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PLCG 2/PLCG2 using anti-PLCG 2/PLCG2 antibody (A03572-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLCG 2/PLCG2 antigen affinity purified polyclonal antibody (Catalog # A03572-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLCG 2/PLCG2 at approximately 148 kDa. The expected band size for PLCG 2/PLCG2 is at 148 kDa.
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