Untreated (–) and treated (+) Raji whole cell extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PLCG2 (phospho Tyr759) antibody [HL3329] (GTX641094) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![Specificity test was performed by coating the plate with PLCG2 peptide and PLCG2 (phospho Tyr759) peptide (250 ng/mL). Coated peptides were probed with PLCG2 (phospho Tyr759) antibody [HL3329] (GTX641094) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641094/GTX641094_T-45551_20241101_ELISA_Indirect_Bar_24110700_418.webp "Specificity test was performed by coating the plate with PLCG2 peptide and PLCG2 (phospho Tyr759) peptide (250 ng/mL). Coated peptides were probed with PLCG2 (phospho Tyr759) antibody [HL3329] (GTX641094) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
Untreated (–) and treated (+) Raji whole cell extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PLCG2 (phospho Tyr759) antibody [HL3329] (GTX641094) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
PLCG2 (phospho Tyr759) antibody [HL3329]
GTX641094
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPLCG2
Overview
- SupplierGeneTex
- Product NamePLCG2 (phospho Tyr759) antibody [HL3329]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3329
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5336
- Target namePLCG2
- Target descriptionphospholipase C gamma 2
- Target synonymsAPLAID, FCAS3, PLC-IV, PLC-gamma-2, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2, phosphoinositide phospholipase C-gamma-2, phospholipase C, gamma 2 (phosphatidylinositol-specific), phospholipase C-IV
- HostRabbit
- IsotypeIgG
- Protein IDP16885
- Protein Name1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
- Scientific DescriptionThe protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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