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ProductsBack/PLD2 / Phospholipase D2 Antibody

PLD2 / Phospholipase D2 Antibody

LS-C496900
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPLD2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PLD2 / Phospholipase D2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 38kDa/104kDa/105kDa, while the observed MW by Western blot was 110kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:3000) The predicted MW is 38kDa/104kDa/105kDa, while the observed MW by Western blot was 110kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    4.28 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5338
  • Target name
    PLD2
  • Target description
    phospholipase D2
  • Target synonyms
    PLD1C, phospholipase D2, choline phosphatase 2, phosphatidylcholine-hydrolyzing phospholipase D2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLD2 using anti-PLD2 antibody (A02586). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLD2 antigen affinity purified polyclonal antibody (Catalog # A02586) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLD2 at approximately 106KD. The expected band size for PLD2 is at 95KD.
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