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ProductsBack/PLEKHG4 Rabbit pAb

PLEKHG4 Rabbit pAb

A16112
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetPLEKHG4
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Overview

  • Supplier
    ABclonal
  • Product Name
    PLEKHG4 Rabbit pAb
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25894
  • Target name
    PLEKHG4
  • Target description
    pleckstrin homology and RhoGEF domain containing G4
  • Target synonyms
    ARHGEF44, PRTPHN1, SCA4, puratrophin-1, PH domain-containing family G member 4, Purkinje cell atrophy associated protein 1, pleckstrin homology domain containing, family G (with RhoGef domain) member 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ58EX7
  • Protein Name
    Puratrophin-1
  • Reactivity
    Human, Rat
  • Reactivity Supplier Note
    Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLEKHG4 using anti-PLEKHG4 antibody (A10252-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLEKHG4 antigen affinity purified polyclonal antibody (Catalog # A10252-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLEKHG4 at approximately 140 kDa. The expected band size for PLEKHG4 is at 131 kDa.
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