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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA020661(PLIN1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA020661(PLIN1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA020661(PLIN1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)

PLIN1 Antibody

CSB-PA020661
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPLIN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PLIN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5346
  • Target name
    PLIN1
  • Target description
    perilipin 1
  • Target synonyms
    FPLD4, PERI, PLIN, perilipin-1, lipid droplet-associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60240
  • Protein Name
    Perilipin-1
  • Scientific Description
    The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5 UTR, but encoding the same protein, have been found for this gene.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Perilipin A using anti-Perilipin A antibody (A03918-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Perilipin A antigen affinity purified polyclonal antibody (Catalog # A03918-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Perilipin A at approximately 62KD. The expected band size for Perilipin A is at 56KD.
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Lane 1: Mouse Thymus lysates probed with Perilipin A Polyclonal Antibody, Unconjugated (bs-3789R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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