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ProductsBack/PLK1 / PLK-1 Antibody (clone 1D1)

PLK1 / PLK-1 Antibody (clone 1D1)

LS-C169107
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetPLK1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PLK1 / PLK-1 Antibody (clone 1D1)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), IF (1:50 - 1:200), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1D1
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5347
  • Target name
    PLK1
  • Target description
    polo like kinase 1
  • Target synonyms
    cell cycle regulated protein kinase; PLK; PLK-1; polo (Drosophia)-like kinase; serine/threonine-protein kinase 13; serine/threonine-protein kinase PLK1; STPK13
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLK1 using anti-PLK1 antibody (PB9500). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40ug, Lane 2: PANC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLK1 antigen affinity purified polyclonal antibody (Catalog # PB9500) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLK1 at approximately 68 kDa. The expected band size for PLK1 is at 68 kDa.
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