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Western Blot analysis of MCF-7 cells using Snk Polyclonal Antibody
Western Blot analysis of MCF-7 cells using Snk Polyclonal Antibody
Western Blot analysis of MCF-7 cells using Snk Polyclonal Antibody

PLK2 Antibody

CSB-PA004124
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPLK2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PLK2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10769
  • Target name
    PLK2
  • Target description
    polo like kinase 2
  • Target synonyms
    SNK, hPlk2, hSNK, serine/threonine-protein kinase PLK2, PLK-2, serine/threonine-protein kinase SNK, serum-inducible kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NYY3
  • Protein Name
    Serine/threonine-protein kinase PLK2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLK2 using anti-PLK2 antibody (A02881). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 cell lysate, Lane 2: human HepG2 cell lysate, Lane 3: mouse heart tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLK2 antigen affinity purified polyclonal antibody (Catalog # A02881) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLK2 at approximately 78KD. The expected band size for PLK2 is at 78KD.
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