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Western blot All lanes: PLLP antibody at 4ug/ml Lane 1: Mouse gonadal tissue Lane 2: Mouse kidney tissue Lane 3: Mouse lung tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa
Western blot All lanes: PLLP antibody at 4ug/ml Lane 1: Mouse gonadal tissue Lane 2: Mouse kidney tissue Lane 3: Mouse lung tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa
Western blot All lanes: PLLP antibody at 4ug/ml Lane 1: Mouse gonadal tissue Lane 2: Mouse kidney tissue Lane 3: Mouse lung tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa

PLLP Antibody

CSB-PA896501LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetPLLP
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Overview

  • Supplier
    Cusabio
  • Product Name
    PLLP Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51090
  • Target name
    PLLP
  • Target description
    plasmolipin
  • Target synonyms
    plasma membrane proteolipid (plasmolipin); plasmolipin; PMLP; TM4SF11; transmembrane 4 superfamily member 11 (plasmolipin)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y342
  • Protein Name
    Plasmolipin
  • Scientific Description
    Appears to be involved in myelination. Could also participate in ion transport events as addition of plasmolipin to lipid bilayers induces the formation of ion channels, which are voltage-dependent and K(+)-selective.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Plasmolipin/PLLP using anti-Plasmolipin/PLLP antibody (A10793). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plasmolipin/PLLP antigen affinity purified polyclonal antibody (Catalog # A10793) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plasmolipin/PLLP at approximately 26 kDa. The expected band size for Plasmolipin/PLLP is at 18,20 kDa.
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