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Western Blot analysis of 3T3 cells using CD232 Polyclonal Antibody
Western Blot analysis of 3T3 cells using CD232 Polyclonal Antibody
Western Blot analysis of 3T3 cells using CD232 Polyclonal Antibody

PLXNC1 Antibody

CSB-PA006287
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPLXNC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PLXNC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10154
  • Target name
    PLXNC1
  • Target description
    plexin C1
  • Target synonyms
    CD232, PLXN-C1, VESPR, plexin-C1, plexin (semaphorin receptor), receptor for viral semaphorin protein, receptor for virally-encoded semaphorin, virus-encoded semaphorin protein receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60486
  • Protein Name
    Plexin-C1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLXNC1 using anti-PLXNC1 antibody (A10941-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLXNC1 antigen affinity purified polyclonal antibody (Catalog # A10941-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLXNC1 at approximately 220 kDa. The expected band size for PLXNC1 is at 176 kDa.
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