![IHC-P analysis of human brain tissue using GTX22783 PMCA4 ATPase antibody [JA9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100 IHC-P analysis of human brain tissue using GTX22783 PMCA4 ATPase antibody [JA9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX22783/GTX22783_1103_IHC-P_w_23060620_571.webp)
IHC-P analysis of human brain tissue using GTX22783 PMCA4 ATPase antibody [JA9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
PMCA4 ATPase antibody [JA9]
GTX22783
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityAmphibian, Bovine, Human, Porcine, Rat
TargetATP2B4
Overview
- SupplierGeneTex
- Product NamePMCA4 ATPase antibody [JA9]
- Delivery Days Customer9
- Antibody SpecificityThe epitope for this antibody has been mapped to amino acids 51-75 of PMCA4 ATPase. This sequence is common to both PMCA4a and 4b ATPase.
- Application Supplier NoteWB: 1:1,000. IHC-P: 1:100-1:500. IHC-Fr: 1:100-1:500. FACS: 2microg per 106 cells. ELISA: 1:15,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDJA9
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID493
- Target nameATP2B4
- Target descriptionATPase plasma membrane Ca2+ transporting 4
- Target synonymsATP2B2; ATPase, Ca++ transporting, plasma membrane 4; matrix-remodeling-associated protein 1; MXRA1; plasma membrane calcium-transporting ATPase 4; PMCA4; PMCA4b; PMCA4x; sarcolemmal calcium pump
- HostMouse
- IsotypeIgG1
- Protein IDP23634
- Protein NamePlasma membrane calcium-transporting ATPase 4
- Scientific DescriptionThe protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
- ReactivityAmphibian, Bovine, Human, Porcine, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203