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IHC-P analysis of human brain tissue using GTX22783 PMCA4 ATPase antibody [JA9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
IHC-P analysis of human brain tissue using GTX22783 PMCA4 ATPase antibody [JA9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
IHC-P analysis of human brain tissue using GTX22783 PMCA4 ATPase antibody [JA9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100

PMCA4 ATPase antibody [JA9]

GTX22783
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityAmphibian, Bovine, Human, Porcine, Rat
TargetATP2B4
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Overview

  • Supplier
    GeneTex
  • Product Name
    PMCA4 ATPase antibody [JA9]
  • Delivery Days Customer
    9
  • Antibody Specificity
    The epitope for this antibody has been mapped to amino acids 51-75 of PMCA4 ATPase. This sequence is common to both PMCA4a and 4b ATPase.
  • Application Supplier Note
    WB: 1:1,000. IHC-P: 1:100-1:500. IHC-Fr: 1:100-1:500. FACS: 2microg per 106 cells. ELISA: 1:15,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    JA9
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID493
  • Target name
    ATP2B4
  • Target description
    ATPase plasma membrane Ca2+ transporting 4
  • Target synonyms
    ATP2B2; ATPase, Ca++ transporting, plasma membrane 4; matrix-remodeling-associated protein 1; MXRA1; plasma membrane calcium-transporting ATPase 4; PMCA4; PMCA4b; PMCA4x; sarcolemmal calcium pump
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP23634
  • Protein Name
    Plasma membrane calcium-transporting ATPase 4
  • Scientific Description
    The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  • Reactivity
    Amphibian, Bovine, Human, Porcine, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203